Baculovirus vector as an efficient gene expression system.
نویسندگان
چکیده
منابع مشابه
Baculovirus as mammalian cell expression vector for gene therapy: an emerging strategy.
The monopoly of insect cells to host baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a eukaryotic gene expression system has been shattered with the growing evidence that it also infects mammalian cells in culture. Although AcMNPV fails to replicate in vertebrate cells, it does express alien genes with levels of expression that are dependent on the strength of...
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The Bombyx mori decapentaplegic gene is one of the conserved genes in vertebrate and invertebrates. The TGF-β superfamily contains conserved polypeptide growth factors that play important roles in different cellular processes such as proliferation, apoptosis, differentiation and cell-fate determination. The B. mori dpp gene shares genetic homology with hBMPs and Drosophila dpp. Until now, only ...
متن کاملThe baculovirus vector system for gene delivery into hepatocytes
Gene therapy in the liver requires powerful vectors capable of mediating sufficient gene delivery and expression in affected hepatocytes. Viral vectors are amongst the most efficient tools for gene delivery, and the search for tissue-specific infecting viruses is important for the development of in v i v o gene therapy strategies. We have recently shown for the first time that a genetically mod...
متن کاملBaculovirus-mediated gene transfer in butterfly wings in vivo: an efficient expression system with an anti-gp64 antibody
BACKGROUND Candidate genes for color pattern formation in butterfly wings have been known based on gene expression patterns since the 1990s, but their functions remain elusive due to a lack of a functional assay. Several methods of transferring and expressing a foreign gene in butterfly wings have been reported, but they have suffered from low success rates or low expression levels. Here, we de...
متن کاملForeign gene expression by a baculovirus vector with an expanded host range.
A nuclear polyhedrosis virus (NPV) (Baculoviridae)-based gene expression system was improved by DNA recombination. The BmN cell line established from Bombyx mori and the Sf21 cell line established from Spodoptera frugiperda are non-permissive for Autographa californica multicapsid NPV (AcMNPV) and B. mori NPV (BmNPV) replication, respectively. After cotransfection of AcMNPV DNA and BamHI-digest...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 1993
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.33.295